Differential gene expression pattern of mouse livers transfected with dual oncogenes (day-7 post-injection data set). Differential gene expression pattern of mouse livers transfected with dual oncogenes (day-7 post-injection data set)

To understand global gene expression patterns of mouse livers upon dual-oncogene transfection, RNA sequencing and analysis were conducted on liver samples from Shp2F/F or Shp2∆H (Shp2F/F:Alb-cre) mice hydrodynamically injected with the combination of Met/β-Catenin or Met/PIK3CA . Overall design: 18 mice from 6 experimental groups (n=3 for each group) were included in the experiment. Group1: GFP hydrodynamically injected Shp2F/F control mice; Group2: GFP hydrodynamically injected Shp2ΔH control mice; Group3: Met/β-Catenin hydrodynamically injected Shp2F/F mice; Group4: Met/β-Catenin hydrodynamically injected Shp2ΔH mice; Group5: Met/PIK3CA hydrodynamically injected Shp2F/F mice; Group6: Met/PIK3CA hydrodynamically injected Shp2ΔH mice. Total RNA was extracted on day-7 post-injection from liver of each mouse.

为探究双癌基因转染后小鼠肝脏的全局基因表达谱，本研究对经水动力注射携带Met/β-连环蛋白（β-Catenin）或Met/PIK3CA组合的Shp2F/F或Shp2ΔH（Shp2F/F:Alb-cre）小鼠的肝脏样本开展了RNA测序及分析。
实验总体设计：本实验共纳入6个实验组，每组生物学重复数为3（n=3），共计18只小鼠。各组设置如下：
组1：经水动力注射GFP的Shp2F/F对照小鼠；
组2：经水动力注射GFP的Shp2ΔH对照小鼠；
组3：经水动力注射Met/β-连环蛋白的Shp2F/F小鼠；
组4：经水动力注射Met/β-连环蛋白的Shp2ΔH小鼠；
组5：经水动力注射Met/PIK3CA的Shp2F/F小鼠；
组6：经水动力注射Met/PIK3CA的Shp2ΔH小鼠。
所有小鼠均于注射后第7天采集肝脏组织并提取总RNA。