Cardio-immune Interaction via a Clusterin-TLR4 Axis Suppresses Inflammation and Triggers Regeneration

This study aimed to investigate the transcriptional effects of BMP2 treatment on neonatal mouse cardiomyocytes. Primary cardiomyocytes were treated with either PBS or BMP2 and subjected to bulk RNA sequencing to profile BMP2-induced changes in gene expression. To further dissect the underlying signaling mechanisms, cells were co-treated with the BMP2 receptor inhibitor Dorsomorphin, allowing assessment of the contribution of the canonical SMAD pathway to BMP2-mediated transcriptional regulation. Comparative transcriptomic analysis across treatment groups provides new insights into the role of the BMP2–SMAD signaling axis in cardiomyocyte gene regulation. Overall design: To investigate the transcriptional effects of BMP2 treatment on neonatal mouse cardiomyocytes, we performed bulk RNA-seq on CMs treated with either BMP2 or PBS. In addition, cells were treated with the BMP2 receptor inhibitor Dorsomorphin to assess the contribution of the downstream canonical SMAD signaling pathway to gene expression.

本研究旨在探究骨形态发生蛋白2（BMP2）处理对新生小鼠心肌细胞的转录调控效应。将原代心肌细胞分别经磷酸盐缓冲液（PBS）或BMP2处理后，进行批量RNA测序（bulk RNA sequencing），以解析BMP2诱导的基因表达变化。为进一步解析潜在的信号转导机制，本研究同时使用BMP2受体抑制剂多索莫德（Dorsomorphin）对细胞进行共处理，以此评估经典SMAD通路在BMP2介导的转录调控中的作用。不同处理组间的比较转录组学分析，为阐明BMP2–SMAD信号轴在心肌细胞基因调控中的功能提供了全新见解。整体实验设计：为探究BMP2处理对新生小鼠心肌细胞的转录调控效应，本研究对分别经BMP2或PBS处理的心肌细胞进行了批量RNA测序（RNA-seq）。此外，为评估下游经典SMAD信号通路对基因表达的贡献，研究同时设置了BMP2受体抑制剂多索莫德处理组。