The inhibitor protein IF1 from mammalian mitochondria inhibits ATP hydrolysis but not ATP synthesis by the ATP synthase complex

The hydrolytic activity of the ATP synthase in bovine mitochondria is inhibited by a protein called IF1, but bovine IF1 has no effect on the synthetic activity of the bovine enzyme in mitochondrial vesicles in the presence of a proton motive force. As part of an investigation into the inhibitory effects on the hydrolytic and synthetic activities of ATP synthase in bovine sub-mitochondrial particles, the absolute quantities of both the ATP synthase complex and the IF1 protein have been measured.

牛线粒体中的ATP合酶（ATP synthase）水解活性可被一种名为IF1的蛋白质抑制。但在质子动力势（proton motive force）存在的情况下，牛源IF1对线粒体囊泡中该牛源ATP合酶的合成活性无影响。作为对牛亚线粒体颗粒中ATP合酶水解与合成活性抑制效应开展研究的一部分，本研究已完成ATP合酶复合物与IF1蛋白的绝对定量测定。