Methylated DNA immunoprecipitation coupled with NimbleGen's array to identify methylated genes in pancreatic ductal adenocarcinoma

Pancreatic ductal adenocarcinoma (PDAC) is a highly invasive cancer with a poor prognosis. Using methylated DNA immunoprecipitation (MeDIP)-chip analysis, we found that 161 genes that were specifically hypermethylated in PANC-1 cells. Among them, miR-615-5p was hypermethylated in its putative promoter region, which silenced its expression in PDAC cell lines. Comparison between PANC-1 cell lines and normal pancreas tissue

胰腺导管腺癌（Pancreatic ductal adenocarcinoma, PDAC）是一类侵袭性极强、预后极差的恶性肿瘤。本研究通过甲基化DNA免疫沉淀（MeDIP）芯片分析，在PANC-1细胞中发现161个特异性高甲基化基因。其中，miR-615-5p的推定启动子区域发生高甲基化，进而沉默其在胰腺导管腺癌细胞系中的表达。本研究对PANC-1细胞系与正常胰腺组织进行了对比分析。