Studying relative gene expression in Head and Neck (HNSCC) tumor under diverse culture conditions

To compare the gene expression profile in HNSCC tumor cultured under various conditions Primary human HNSCC tumor was cultured for upto 72 hours in presence of Autologous Serum and combination of Autologous Serum and Tissue metalloproteinases ; angiogeneesis and tissue associated microenvironment signatures were studied

为对比不同培养条件下的人头颈部鳞状细胞癌（Head and Neck Squamous Cell Carcinoma，HNSCC）实体瘤基因表达谱，研究人员将原代人HNSCC实体瘤分别在添加自体血清（Autologous Serum）、自体血清联合组织金属蛋白酶（Tissue metalloproteinases）的培养体系中培养至多72小时，并对其血管生成及组织相关微环境特征展开了研究。