PMC exhibits less sensitivity to decreasing signal-to-noise ratio (SNR)

The image data for Figure 7, and Figures S6 of Redefining colocalization analysis with a novel Phasor Mixing Coefficient.This contains multispectral imaging data of Ptk2 cells in which non-muscle myosin II heavy chain (MHC) was labeled with AlexaFluor(AF)-488, while its phosphorylated regulatory light chain (RLC) was labelled with AF-568.These data reflect measuring PMC across a range of SNR's which were produced by imaging the cells at increasing laser power on both lines.The folders "greenonly_cell1" and "redonly_cell1" contain the single-color controls; the folders "dualcolor_cell3", "dualcolor_cell4", and "dualcolor_cell5" contain the two-color experiments.For each image file, the filename contains the relevant laser power information. After a preamble of "Ptk_..." which identifies whether MHC, RLC, or both are labelled and the experiment number, the names contain a sequence such as "0p01-488_0p1-561". This indicates the laser power settings for that image: in this case, the 488nm laser was set to 0.01% and the 561nm laser was set to 0.1. The six combinations are as follows.488: 0.01, 0.06, 0.12, 0.18, 0.24, 0.3%561: 0.1, 0.3, 0.6, 0.9, 1.2, 1.5%The analysis outputs are produced through the "Figure 7" section of the Jupyter notebook in the associated GitHub repository.本数据集包含论文《利用新型相量混合系数重新定义共定位分析》（Redefining colocalization analysis with a novel Phasor Mixing Coefficient）中图7及补充图S6所用的图像数据。该数据集涵盖PtK2细胞的多光谱成像数据：其中非肌肉肌球蛋白II重链（non-muscle myosin II heavy chain, MHC）经AlexaFluor-488（AF-488）标记，其磷酸化调节轻链（phosphorylated regulatory light chain, RLC）则经AF-568标记。本研究通过对两条成像通道以递增激光功率成像，获取了覆盖不同信噪比（signal-to-noise ratio, SNR）范围的图像数据，用于计算相量混合系数（Phasor Mixing Coefficient, PMC）。文件夹`greenonly_cell1`与`redonly_cell1`为单色对照样本；文件夹`dualcolor_cell3`、`dualcolor_cell4`及`dualcolor_cell5`为双色实验样本。每张图像文件的文件名均包含对应的激光功率信息：文件名以`Ptk_`作为前缀，用于标识标记物类型（MHC、RLC或二者均标记）及实验编号；其后的序列如`0p01-488_0p1-561`则代表该图像的激光功率设置，此例中488nm激光功率设为0.01%，561nm激光功率设为0.1%。本次实验共设置6组激光功率组合，具体如下： - 488nm激光：0.01%、0.06%、0.12%、0.18%、0.24%、0.3% - 561nm激光：0.1%、0.3%、0.6%、0.9%、1.2%、1.5% 相关分析结果可通过关联GitHub仓库中Jupyter Notebook的「Figure 7」章节生成。