Biochemical and Proteomic proteomic changes in the roots of M4 grapevine rootstock in response to nitrate availability

In this project a proteomic comparative analysis was used to analyse the changes induced by different nitrate availability in roots of M4 grapevine rootstock. Plants, previously adapted to hydroponic condition, were grown for 5 weeks, afterwards nitrogen was removed from the growing medium for eight days to obtain N-starved plants. After this period, the analysis compared plants maintained in absence of nitrogen with plants exposed to 10 mM nitrate for 30 hours.

本研究采用蛋白质组比较分析手段，解析不同硝态氮供给水平对M4葡萄砧木（M4 grapevine rootstock）根系所诱导的蛋白表达变化。预先适应水培条件的植株经5周培养后，将其生长培养基中的氮源移除8天，以获得氮饥饿植株。该预处理阶段结束后，本分析将持续处于无氮环境的植株与经10 mM硝态氮处理30小时的植株进行对照比较。