A protocol for robust discovery proteomics using nano liquid chromatography using pillar-array column technology and high-resolution mass spectrometry with data- independent acquisition

The project establishes an easy-to-follow protocol to set up bottom-up proteomics experiments using state-of-the-art pillar-array columns and mass spectrometry. The protocol touches upon key points of the method development, discusses the trade-off between proteome depth and analysis time and demonstrates short-term robustness.

本项目构建了一套易于遵循的实验方案，用于依托前沿柱阵列色谱柱（pillar-array columns）与质谱法（mass spectrometry）开展自下而上蛋白质组学（bottom-up proteomics）实验。该方案涵盖方法开发的核心要点，探讨了蛋白质组覆盖深度与分析时长之间的权衡关系，并验证了其短期鲁棒性。