mRNA expression in pollen of Prunus avium and Prunus mume

Pollen grains from three cultivars in two Prunus species, P. avium and P. mume, were subjected to mRNAseq analyses by Illumina HiSeq 2000. After trimming procedures, each informative 29,812,682, 35,162,899, 32,728,911 reads for cvs. Satonishiki (P. avium), Kairyo-Uchida-Ume and Nanko (P. mume), respectively, were mapped to predicted mRNA transcripts sequences in the peach genome database (from GDR, Prunus_persica_v1.0) and mRNA contig collections in P. mume database by BWA/Sam tools, and subjected to DEseq analysis in R. Using these results, we grasped general components expressed in pollen of Prunus, and examined differences in expression levels among genes, especially on S-locus-linked F-box (SFB/SLFL) genes and their orthologs, with discussion of their evolutionary path.

以两个李属（Prunus）物种的3个栽培品种的花粉粒为实验材料，采用Illumina HiSeq 2000平台开展mRNA测序（mRNAseq）分析。经序列修剪流程处理后，栽培品种Satonishiki（P. avium）、Kairyo-Uchida-Ume和Nanko（P. mume）的有效测序读段数分别为29,812,682、35,162,899和32,728,911条。随后借助BWA/Sam工具，将这些读段比对至桃基因组数据库（源自GDR，版本为Prunus_persica_v1.0）的预测mRNA转录本序列，以及梅数据库中的mRNA重叠群（contig）集合，并在R语言环境中通过DEseq进行差异表达分析。基于上述分析结果，本研究明确了李属植物花粉中表达的核心基因组分，检测了不同基因的表达水平差异，重点聚焦于S位点关联F-box（S-locus-linked F-box，SFB/SLFL）基因及其直系同源基因，并对其进化路径展开了探讨。