ChIP-seq data of MbpR in Lactiplantibacillus plantarum WCFS1

The WYL transcription factor (MbpR) is a c-di-GMP effector in L. plantarum WCFS1. MbpR binds to a special motif within the coding sequence, impeding the transcriptional elongation of mbpA and mbpB, which encode mucus binding proteins (MucBPs). The perception of c-di-GMP by the WYL domain reversed the inhibitory effect of MbpR on the expression of MucBPs, resulting in increased adherence to intestinal epithelial cells by L. plantarum. Overall, our study provides evidence that a WYL transcription factor participates in probiotic colonization by sensing c-di-GMP.

WYL转录因子（WYL transcription factor，MbpR）是植物乳杆菌（L. plantarum）WCFS1中的环二鸟苷酸（c-di-GMP）效应蛋白。MbpR结合于编码序列内的特异性基序，阻碍mbpA与mbpB的转录延伸，这两个基因编码黏液结合蛋白（MucBPs）。WYL结构域对环二鸟苷酸的感知可逆转MbpR对黏液结合蛋白表达的抑制作用，使植物乳杆菌对肠道上皮细胞的黏附能力得以提升。综上，本研究证实，WYL转录因子可通过感知环二鸟苷酸参与益生菌的定殖过程。