Dectin-1-mediated signaling leads to characteristic gene expressions in rat mast cells. Rattus norvegicus

Rat mast cell line RBL-2H3 was analyzed to investigate the molecular mechanism of Dectin-1-mediated activation and responses of mast cells. Dectin-1-mediated signaling stimulated characteristic gene expression, such as MCP-1, IL-4, IL-13, TNF-αand Nfkbiz. Overall design: RBL-2H3 cell line stably expressing Dectin-1 was established. Non-stimulated cells (control) compared to Dectin-1-stimulated (Curdlan) cells in order to comfirm Dectin-1-mediated signaling-inducible genes. Dectin-1-stimulated cells compared to Syk inhibitor R406-pretreated cells in order to examine the importance of Syk for Dectin-1-mediated gene up-regulations.

本研究以大鼠肥大细胞系RBL-2H3为研究对象，旨在探究Dectin-1（Dectin-1）介导的肥大细胞活化与应答的分子机制。Dectin-1介导的信号通路可诱导特征性基因表达谱，涉及单核细胞趋化蛋白1（MCP-1）、白细胞介素4（IL-4）、白细胞介素13（IL-13）、肿瘤坏死因子α（TNF-α）及Nfkbiz等基因。

实验整体设计如下：首先构建稳定表达Dectin-1的RBL-2H3细胞系。其一，将未受刺激的细胞（对照组）与经凝结多糖（Curdlan）刺激的Dectin-1活化细胞进行比较，以确认Dectin-1介导的信号通路诱导的差异表达基因；其二，将经脾酪氨酸激酶（Syk）抑制剂R406预处理的细胞与Dectin-1刺激后的细胞进行比较，以验证Syk在Dectin-1介导的基因上调过程中的重要作用。