The gene expression of wild-type worms and kin-1(ok338) mutant worms exposing Salmonella entericaSL1344 at 24 hours. Caenorhabditis elegans

PKA/KIN-1 pathway is required for innate immunity in C. elegans, however, the downstream signaling is largely unclear. To gain further insight into mechanism underlying the promoted immunity of PKA/KIN-1 pathway, we performed genome microarrays of wild-type and kin-1(ok338) worms infection by Salmonella entericSL1344, compared with the controls wild-type fed E.coli OP50 Overall design: The gene expression in wild-type worms and kin-1(ok338) mutant worms after exposure Salmonella entericaSL1344 at 24 hours. all experiments were performed three times independently.

PKA/KIN-1通路是秀丽隐杆线虫（C. elegans）先天免疫所必需的，但其下游信号转导机制尚未完全明确。为进一步阐明PKA/KIN-1通路介导免疫增强的潜在分子机制，我们对感染肠炎沙门氏菌SL1344（Salmonella enterica SL1344）的野生型线虫及kin-1(ok338)突变型线虫开展了基因组微阵列分析，并以喂食大肠杆菌OP50（E. coli OP50）的野生型线虫作为对照。实验整体设计：检测暴露于肠炎沙门氏菌SL1344 24小时后，野生型线虫与kin-1(ok338)突变型线虫的基因表达水平。所有实验均独立重复三次。