microRNA expression profiling in human gastric cancer

Using the highly sensitive miRCURY LNA™ microRNA array, we screened 3100 microRNAs abundant in the human gastric cancer and Adjacent normal gastric mucosa tissues, and the function of differentially expressed microRNAs were analyzed by bioinformatics. The enrichment results indicated that these microRNAs perhaps participated in the occurrence and development process of gastric cancer. In this study, three cases of gastric cancer were used to acquire the microRNA expression profiling, and qPCR was employed to confirm the results of microRNA chip. The function of the differentially expressed microRNA were analyzed by bioinformatic methods. Finally,compared with the adjacent mormal moucosa tissues，the expression of 99 microRNAs were up regulated,and 21 microRNAS were down regulated in gastric cancer.qPCR results showed an expression pattern consistent with that of the chip analysis.

本研究采用高灵敏度的miRCURY LNA™微RNA（microRNA）芯片，对人胃癌及癌旁正常胃黏膜组织中富集的3100种微RNA进行筛选，并通过生物信息学方法分析差异表达微RNA的功能。富集分析结果显示，此类微RNA可能参与胃癌的发生与发展进程。本研究选取3例胃癌组织样本获取微RNA表达谱，并采用实时定量聚合酶链反应（qPCR）验证微RNA芯片的检测结果，再次通过生物信息学方法分析差异表达微RNA的功能。最终，与癌旁正常黏膜组织相比，胃癌组织中共有99种微RNA呈上调表达，21种微RNA呈下调表达；qPCR验证结果与芯片分析的表达模式一致。