Expression Array analysis of MTAP in HT080 cells

Methylthioadenosine Phosphorylase (MTAP) is a tumor suppressor gene that encodes an enzyme responsible for the catabolism of the polyamine byproduct 5′deoxy-5′-methylthioadenosine (MTA). To elucidate the mechanism by which MTAP inhibits tumor formation, we have created isogenic MTAP+ and MTAP- HT1080 fibrosarcoma cells. In this experiment we have performed expression array analysis on MTAP-, MTAP+, and MTAP+ cells treated with the MTAP inhibitor MT-DADMe-ImmA. Three biological replicates of each sample were grown and analyzed. M- is MTAP-. M+ is MTAP+, and M+I is MTAP treated with inhibitor (48 hours).

甲硫腺苷磷酸化酶（Methylthioadenosine Phosphorylase, MTAP）是一种抑癌基因，其编码的酶负责多胺副产物5'-脱氧-5'-甲硫腺苷（5′deoxy-5′-methylthioadenosine, MTA）的分解代谢。为阐明MTAP抑制肿瘤形成的分子机制，我们构建了同基因背景的MTAP阳性（MTAP+）与MTAP阴性（MTAP-）HT1080纤维肉瘤细胞系。本实验针对MTAP-、MTAP+以及经MTAP抑制剂MT-DADMe-ImmA处理的MTAP+细胞开展了基因表达阵列分析。每个样本均设置3次生物学重复，并完成培养与分析。其中，M-指代MTAP-细胞，M+指代MTAP+细胞，M+I指代经抑制剂处理48小时的MTAP+细胞。