Accelerated Healing Period with Hydrophilic Implant Placed in Sheep Tibia

Abstract The objective of this study was to evaluate the early osseointegration of two different implants surfaces, a sandblasted and acid-etched surface (TN) compared with same geometry and surface roughness modified to be hydrophilic/wettable by conditioning in an isotonic solution of 0.9% sodium-chloride (TA) through histological and histomorphometric analysis after sheep tibia implantation. Forty dental implants, divided in two groups (TN and TA) were placed in the left tibia of twenty healthy, skeletally mature Santa Ines sheep (n=5/experimental period). After 7, 14, 21 and 28 days post-implantation, the samples were removed and the sheep were kept alive. Analysis of resin sections (30 μm) allowed the quantification of bone area (BA) and bone-to-implant contact (BIC). TA group presented nearly 50% increase in BA at 14 days (p<0.001, ANOVA - Tukey’s post test) compared with 7 days. The TA presented higher values than the TN for BA and BIC at 14, 21, and 28 days after placement, stabilizing bone healing. TA hydrophilic surface promoted early osseointegration at 14 and 21 days compared to TN, accelerating bone healing period post-implant placement in sheep tibia.

摘要 本研究旨在评估两种不同种植体表面的早期骨整合（osseointegration）效果：喷砂酸蚀表面（TN），以及采用0.9%氯化钠等渗溶液浸润改性为亲水/可湿表面、且几何形态与表面粗糙度与前者一致的种植体表面（TA），通过绵羊胫骨植入术后的组织学与组织形态计量学分析进行对比。本研究纳入20只健康、骨骼发育成熟的圣塔伊内斯绵羊（每实验周期n=5），于其左侧胫骨植入40枚牙科种植体（dental implants），分为TN组与TA组两组。于种植术后7、14、21及28天采集样本并移除种植体，期间绵羊保持存活。通过对30μm树脂切片的分析，可量化骨面积（BA）与种植体-骨接触面积（BIC）。与术后7天相比，TA组在术后14天时的骨面积（BA）提升近50%（p<0.001，方差分析（ANOVA）- Tukey事后检验）。在种植体植入后的14、21及28天，TA组的骨面积与种植体-骨接触面积（BIC）均高于TN组，骨愈合过程趋于稳定。相较于TN组，TA亲水表面可在术后14天与21天促进早期骨整合，加速绵羊胫骨种植术后的骨愈合周期。