Expression data from human foetal neural stem (NS) cell lines and human glioma neural stem (GNS) cell lines

Gliomas have been proposed to be driven by a population of neural stem-like cells. We isolated a panel of novel human glioma cell lines using adherent neural stem cell conditions. The normal human foetal (hf) NS cells and the tumorigenic glioma NS cell lines were expanded using growth factors EGF and FGF in adherent culture conditions. In these conditions apoptosis and differentiation are suppressed resulting in more homogeneous populations of stem cells than has been reported previously. We included parallel primary biopsies of non-malignant brain tissue ('Normal Brain'). Cell lines were expanded until 70-90% confluent and then harvested for RNA extraction. All cell lines were gorwn in identical culture media. We also include 'Normal Brain' samples which are regions of the adult human cortex.

已有研究提出，神经胶质瘤（glioma）的发生发展由一类神经干细胞样细胞群体驱动。本研究采用贴壁神经干细胞培养体系，成功分离得到一系列新型人源胶质瘤细胞系。正常人类胎儿源（human foetal, hf）神经干细胞（NS）以及致瘤性胶质瘤NS细胞系，均在贴壁培养条件下，借助表皮生长因子（EGF）和成纤维细胞生长因子（FGF）进行扩增培养。在此培养体系中，细胞凋亡与分化进程均受到抑制，由此获得的干细胞群体均质性显著优于既往报道的结果。本研究同时纳入了非恶性脑组织的平行原代活检样本，命名为"Normal Brain"。细胞系扩增至汇合度70%-90%时，收集样本用于RNA提取。所有细胞系均采用完全一致的培养基进行培养。此外本研究还纳入了取自成人大脑皮层区域的"Normal Brain"样本。