Host transcriptome profiling analysis induced byMd5BAC, Md5BAC △LORF9, Md5BAC △UL23,Md5BAC △LORF9-Re, Md5BAC △UL23-Re reconbinant viruses.

Marek's disease virus (MDV) causes immunosuppression and tumor diseases in chickens. MDV encodes more than 100 genes, most of which are homologous to HSV-1 genes, and are mainly involved in viral genome DNA replication, virus particle assembly and morphogenesis. Although the functions of some genes have been analyzed, many genes are unknown because of the huge genome of MDV. In order to explore the pathogenesis of LORF9 and UL23 in vivo, LORF9 and UL23 gene deletion strains were constructed. Gene expression profiles of recombinant virus and Md5BAC wild strains were analyzed by RNA-seq data. To clarify the gene expression level of recombinant virus in the early stage of cytolytic infection. Comparative gene expression profiling analysis of RNA-seq data for Md5BAC and 4 recombinant viruses ( Md5BAC△LORF9, Md5BAC△ UL23, Md5BAC△LORF9-Re, Md5BAC△ UL23-Re).

马立克氏病病毒（Marek's disease virus，MDV）可导致鸡群出现免疫抑制与肿瘤性疾病。该病毒编码百余种基因，其中多数与单纯疱疹病毒1型（Herpes simplex virus 1, HSV-1）基因同源，主要参与病毒基因组DNA复制、病毒颗粒组装与形态发生过程。尽管已有部分基因的功能得到解析，但由于MDV基因组庞大，仍有大量基因的功能尚未明确。为探究LORF9与UL23基因在体内的致病机制，本研究构建了LORF9基因缺失株与UL23基因缺失株。通过RNA测序（RNA sequencing, RNA-seq）数据分析重组病毒与Md5BAC野生毒株的基因表达谱，以明确裂解感染早期阶段重组病毒的基因表达水平。对Md5BAC毒株与4株重组病毒（Md5BAC△LORF9、Md5BAC△UL23、Md5BAC△LORF9-Re、Md5BAC△UL23-Re）的RNA-seq数据开展比较基因表达谱分析。