Diffuse large B-cell lymphoma gene expression profiling array VFN-D2.. Homo sapiens

The dataset contains data from 12 samples hybridized on Illumina HumanHT-12 array. -patient's primary lymphoma cells obtained from malignant ascites (P2-A1, P2-A2) -patient's non-malignant B-cells (CTRL1, CTRL2) -lymphoma cell line named UPF4D established from the patient's primary lymphoma cells after 15 and 75 days of in vitro cultivation (UPF4D-D15A, UPF4D-D15B, UPF4D-D75) -cells established by xenotransplantation of UPF4D cell line isolated ex vivo from infiltrated murine kidney (UPF4D-K) -cells established by xenotransplantation of UPF4D cell line isolated ex vivo from subcutaneously growing lymphoma (UPF4D-SC) -patient-derived xenograft (PDX) cells named VFN-D2 established by xenotransplantation of primary lymphoma cells into immunodeficient mice isolated ex vivo from the infiltrated murine kidneys (VFN-D2-K1, VFN-D2-K2) -patient-derived xenograft (PDX) cells named VFN-D2 established by xenotransplantation of primary lymphoma cells into immunodeficient mice isolated ex vivo from subcutaneously growing lymphoma (VFN-D2-SC). Samples were sorted using CD19-microbeads (Miltenyi). For xenotransplantation, NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ mice (referred to as NSG mice) were used. Overall design: Unsummarized data can be obtained using R package klenermicroarraydata available from https://bitbucket.org/kulvait/klenermicroarraydata.

本数据集包含12份于Illumina HumanHT-12基因表达芯片（Illumina HumanHT-12 array）上完成杂交的样本，具体如下：
- 取自恶性腹水的患者原发性淋巴瘤细胞（P2-A1、P2-A2）
- 患者非恶性B细胞（CTRL1、CTRL2）
- 经体外培养15天与75天后，由患者原发性淋巴瘤细胞构建的淋巴瘤细胞系UPF4D，对应样本为UPF4D-D15A、UPF4D-D15B、UPF4D-D75
- 将UPF4D细胞系异种移植至免疫缺陷小鼠体内后，从浸润的小鼠肾脏中离体分离得到的细胞（UPF4D-K）
- 将UPF4D细胞系异种移植至免疫缺陷小鼠体内后，从皮下生长的淋巴瘤中离体分离得到的细胞（UPF4D-SC）
- 将患者原发性淋巴瘤细胞异种移植至免疫缺陷小鼠体内构建的患者来源异种移植瘤（Patient-Derived Xenograft，PDX）模型VFN-D2，从浸润的小鼠肾脏中离体分离得到的细胞（VFN-D2-K1、VFN-D2-K2）
- 同样由患者原发性淋巴瘤细胞异种移植构建的VFN-D2模型，从皮下生长的淋巴瘤中离体分离得到的细胞（VFN-D2-SC）

所有样本均通过CD19磁珠（Miltenyi）完成分选。异种移植实验使用NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ小鼠（简称NSG小鼠）。实验整体设计：未汇总的原始数据可通过R语言包klenermicroarraydata获取，下载地址为https://bitbucket.org/kulvait/klenermicroarraydata。