Evaluating levels of Vaccinia viral mRNA translation

We have sequenced polysome enriched mRNA to determine levels of viral transcript translation. Overall design: Comparative gene expression profiling analysis of RNA-seq data enriched for polysomes in Vaccinia infected cells.我们对富集了多聚核糖体（polysome）的信使核糖核酸（mRNA）进行了测序，以测定病毒转录本的翻译水平。实验整体设计：对痘苗病毒（Vaccinia）感染细胞中富集了多聚核糖体的RNA测序（RNA-seq）数据开展比较基因表达谱分析。