Chemical-based external stimulation reprograms human somatic cells into pluripotency (single cell RNA-seq)

Here, we report that chemical-based external stimulation reprograms human somatic cells to generate human induced pluripotent stem cells. Using transcriptome, chromatin accessibility and DNA methylome profiling, we identified the cells exhibited key features of embryonic stem cells in transcriptomic profiles, epigenetic status. In addition, we also analyzed the reprogramming process. To dissect the molecular mechanism, the reprogramming process were analyzed by single cell RNA-seq.

本研究报道，借助基于化学试剂的外源刺激可将人类体细胞重编程为人诱导多能干细胞（human induced pluripotent stem cells）。本研究通过转录组（transcriptome）、染色质开放性（chromatin accessibility）与DNA甲基化组（DNA methylome）测序分析，证实所获细胞在转录组特征及表观遗传状态层面均具备胚胎干细胞（embryonic stem cells）的核心特征。此外，本研究对该重编程过程开展了初步分析；为解析其分子机制，研究团队进一步通过单细胞RNA测序（single cell RNA-seq）对该重编程进程进行了深入解析。