Soil fungal communities in the alpine treeline ecotone under contrasting reindeer grazing regimes

In this study we tested how soil fungal community composition, diversity and function depended on tree vicinity and long-term reindeer grazing regime in an alpine treeline ecotone in Northern Scandinavia. The study consisted of eighteen replicates of soil samples collected directly underneath and > 3 m away from mountain birches (Betula pubescens ssp. czerepanovii) in two adjacent 55-year-old grazing regimes with or without summer grazing by reindeer (Rangifer tarandus). Soil sample collection took place in September 2019, and the samples included the upper 5 cm of the soil, including both litter and humus layers. Upon collection, the samples were freeze-dried, homogenized and characterized for fungal community composition in duplicate samples using high-throughput sequencing of ITS2 rDNA region.Analyses of the community data have been published in the article Reindeer control over subarctic treeline alters soil fungal communities with potential consequences for soil carbon storage by Henni Ylanne, Rieke L. Madsen, Carles Castano, Daniel B. Metcalfe and Karina E. Clemmensen in Global Change Biology.

本研究以斯堪的纳维亚北部高山林线交错带为研究区域，探究土壤真菌群落组成、多样性及功能如何受树木邻近位置与长期驯鹿放牧制度的影响。本研究设置了两个相邻且已实施55年的放牧处理组，分别为夏季有驯鹿（Rangifer tarandus）放牧与无放牧两种制度；在每个处理组中，分别于高山桦树（Betula pubescens ssp. czerepanovii）冠层下方以及距其大于3米的位置采集土壤样本，每组设置18次生物学重复。土壤样本采集于2019年9月，采集深度为土壤上层5厘米，涵盖枯落物层与腐殖质层。样本采集后经冷冻干燥、均质化处理，通过ITS2 rDNA区域（ITS2 rDNA region）高通量测序（high-throughput sequencing）对两份平行样本的真菌群落组成进行分析表征。该真菌群落数据分析结果已发表于期刊《Global Change Biology》的论文《Reindeer control over subarctic treeline alters soil fungal communities with potential consequences for soil carbon storage》，作者为Henni Ylanne、Rieke L. Madsen、Carles Castano、Daniel B. Metcalfe与Karina E. Clemmensen。