Next generation sequencing for miRNA profile of bone marrow mesenchymal stem cells through establishing exercise rat model by treadmill running experiment. Next generation sequencing for miRNA profile of bone marrow mesenchymal stem cells through establishing exercise rat model by treadmill running experiment

In this study, we proved the exercise-induced promotion of osteogenic differentiation and inhibition of adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) through establishing exercise animal model by treadmill running experiment. miRNA sequencing analysis identified 16 upregulated and 2 downregulated miRNAs in exercise group. Overall design: Examination of miRNA profile in bone marrow mesenchymal stem cells (BMSCs) of two model.

本研究通过跑台运动实验构建运动动物模型，证实运动可诱导骨髓间充质干细胞（bone marrow mesenchymal stem cells, BMSCs）的成骨分化，并抑制其成脂分化。微小RNA（microRNA, miRNA）测序分析显示，运动组中共鉴定出16个上调miRNA与2个下调miRNA。实验整体设计：对两种模型的骨髓间充质干细胞中的miRNA表达谱进行检测分析。