Targeted suppression of siRNA biogenesis in Arabidopsis pollen [bisulfite-seq]

We used the RNaseIII-like protein RTL1 to suppress siRNA biogenesis specifically in Arabidopsis pollen, and found distinct siRNA subsets produced in the sperm and vegetative cell lineages via the RNA polymerase IV (Pol IV). Whole-genome bisulfite sequencing of Arabidopsis pollen

我们利用类RNase III蛋白RTL1（RNaseIII-like protein RTL1），特异地在拟南芥花粉中抑制小干扰RNA（siRNA）的生物发生，并发现了经由RNA聚合酶IV（Pol IV）在精子与营养细胞谱系中产生的差异化siRNA亚型，同时对拟南芥花粉开展了全基因组亚硫酸氢盐测序。