Genomic copy number variation analysis using 4 x 180K tiling array

CGH analysis of Arabidopsis genome comprising control diploid wild type (col) with progeny of Ex-TAQing treaded (Ex-TAQed) diploid strain. Ex-TAQed plant was introduced of MseI restriction enzyme gene and activation of MseI by heat treated for 3 hours at 33ºC. Analysis of copy number variation using 16 diploids progenies after activation of Ex-TAQing system.

本数据集针对拟南芥基因组开展比较基因组杂交（Comparative Genomic Hybridization，CGH）分析，实验材料包含二倍体野生型哥伦比亚（Col）对照株系，以及经Ex-TAQing处理（Ex-TAQed）的二倍体菌株的子代株系。其中，Ex-TAQed株系携带有MseI限制性内切酶基因，并通过33℃恒温热处理3小时以激活MseI的酶活性。本研究利用该Ex-TAQing系统激活后的16株二倍体子代株系，完成了拷贝数变异（Copy Number Variation）分析。