The effect of NTR-specific shRNAs on DENV replicationa.

aThe production of NS1 antigen, measured using BioRad's Platelia assay kit, served as a marker of DENV replication; data shown are from one representative screening experiment.bThis indicates the location of the 21 nts corresponding to the sense strand of the sh construct-encoded siRNA, on the DENV genome; numbers indicated correspond to DENV-2 NGC strain (Accession number AF038403).cSLA: stem-loop A; 5′ UAR: 5′ upstream AUG region; 3′ conserved sequence; 3′ UAR: 3′ upstream AUG region; 3′ SL: 3′ stem-loop; nr: not reported.dThe percent inhibition was calculated with reference to DENV infectivity (in the presence of transfected sh-scr construct), which was taken as 100%. D-1, D-2, D-3 and D-4 denote DENV-1, DENV-2, DENV-3 and DENV-4, respectively.