The effect of NTR-specific shRNAs on DENV replicationa.

aThe production of NS1 antigen, measured using BioRad's Platelia assay kit, served as a marker of DENV replication; data shown are from one representative screening experiment.bThis indicates the location of the 21 nts corresponding to the sense strand of the sh construct-encoded siRNA, on the DENV genome; numbers indicated correspond to DENV-2 NGC strain (Accession number AF038403).cSLA: stem-loop A; 5′ UAR: 5′ upstream AUG region; 3′ conserved sequence; 3′ UAR: 3′ upstream AUG region; 3′ SL: 3′ stem-loop; nr: not reported.dThe percent inhibition was calculated with reference to DENV infectivity (in the presence of transfected sh-scr construct), which was taken as 100%. D-1, D-2, D-3 and D-4 denote DENV-1, DENV-2, DENV-3 and DENV-4, respectively.a. 采用伯乐（BioRad）公司的Platelia检测试剂盒测定的NS1抗原（NS1 antigen）生成量，可作为登革病毒（DENV）复制的标志物；本次展示的数据来自1次代表性筛选实验。 b. 本标注明确了登革病毒（DENV）基因组上对应短发夹载体（sh construct）编码的小干扰RNA（siRNA）正义链的21个核苷酸（nts）的位置；标注的编号对应登革病毒2型NGC毒株（登录号AF038403）。 c. SLA：茎环A（stem-loop A）；5′ UAR：5′端AUG上游区域（5′ upstream AUG region）；3′保守序列（3′ conserved sequence）；3′ UAR：3′端AUG上游区域（3′ upstream AUG region）；3′ SL：3′茎环（3′ stem-loop）；nr：未报道（not reported）。 d. 抑制百分比以转染阴性对照短发夹载体（sh-scr construct）时的登革病毒（DENV）感染性为基准（设为100%）计算得出。D-1、D-2、D-3、D-4分别指代登革病毒1型、登革病毒2型、登革病毒3型、登革病毒4型。