High throughput targeted RNA sequencing on HepG2 cell line exposed to multiple concentration levels of drug induced liver injury (DILI) compounds at various different time points_part1. High throughput targeted RNA sequencing on HepG2 cell line exposed to multiple concentration levels of drug induced liver injury (DILI) compounds at various different time points_part1

This experiment was carried out to investigate the responses of the human liver model (HepG2) cells upon the exposure of various DILI compounds in multiple severity scenarios by varying the concentration. The cells were seeded in 384 well plate format containing 8000 cell per well with 2 days of resting before exposure. The compounds were exposed for 8 and 24 hours. The cells were lysed and the lysate was collected for high throughput targeted RNA-seq with the human whole genome library. The gene expression profiles were analyzed to identify the modulated responses of the cells. This experiment is part of the TransQST project.

本实验旨在探究人类肝脏模型（HepG2）细胞在通过调整浓度构建的多种严重程度场景下，暴露于各类药物性肝损伤（Drug-Induced Liver Injury, DILI）化合物时的应答反应。实验将细胞以每孔8000个的密度接种于384孔板，静置培养2天后再进行化合物暴露处理。化合物分别与细胞孵育暴露8小时及24小时。随后裂解细胞并收集裂解液，采用人类全基因组文库开展高通量靶向RNA测序（RNA-seq）。对获取的基因表达谱进行分析，以鉴定细胞的调控应答特征。本实验为TransQST项目的组成部分。