Sensitivity Trials.

Sensitivity testing of each cPCR and qPCR marker across a dilution series of genomic DNA. The proportion of detections out of four replicates is noted for each marker at each dilution level. Estimated marker copy numbers per dilution are noted below each set of markers based on averages calculated across all replicates of qPCR sensitivity trials using a plasmid DNA standard. AC markers were tested using SC dilutions. U = Undeterminable copy number.*Variation in amplifications at these levels are likely due to stochasticity of PCR at such low DNA concentrations.Sensitivity Trials.对每一种常规聚合酶链式反应（cPCR）与定量聚合酶链式反应（qPCR）检测标记物，采用基因组DNA梯度稀释系列开展敏感性测试。记录各标记物在每一级稀释梯度下的四次重复实验中的检出占比。基于使用质粒DNA标准品完成的qPCR敏感性试验全部重复实验的平均值，每组标记物下方标注了对应稀释梯度的估算标记物拷贝数。AC标记物采用SC稀释液进行测试。U代表拷贝数无法确定。*该浓度水平下的扩增波动大概率源于极低DNA浓度下PCR反应的随机性。敏感性试验。