Altered 3D chromatin structure permits inversional recombination at the IgH locus

We report that in the absence of intergenic control region 1 (IGCR1), a regulatory sequence that specifies the RAG scanning domain on WT IgH alleles, VH and DH gene segments can recombine with each other by both deletion and inversion of intervening DNA. Chromatin accessibility of different pro-B cell lines were analyzed by ATAC-Seq method

本研究报道，在缺失基因间调控区1（intergenic control region 1, IGCR1）——该序列为野生型（wild type, WT）免疫球蛋白重链（IgH）等位基因上指定RAG扫描结构域的调控序列——的情况下，VH基因片段与DH基因片段可通过间隔DNA的缺失与倒位两种方式发生重组。本研究通过转座酶可及性测序（ATAC-Seq）方法，对不同前B细胞系的染色质可及性进行了分析。