Population genetics and comparative morphology of two serpentine Phlox species

Hybridization between rare and widespread species can result in loss of genetic integrity for the rarer species, which can have management and conservation implications. One rare species, Phlox hirsuta, is a serpentine endemic in northern California, and it frequently co-occurs with a widespread congener, P. speciosa. Putative hybrids were recognized based on intermediate morphology, so the possibility of hybridization was explored using floral morphological and molecular data. Ninety-eight individuals of P. hirsuta and P. speciosa were collected from each of three populations, and floral features were measured and compared. Eleven microsatellite loci were amplified for species and putative hybrids, and inter- and intraspecific genetic diversity and relationships were investigated with multiple methods. Variation in morphological and molecular data was recognized. Floral variation was greater for P. hirsuta than P. speciosa. Putative hybrids were genetically allied with P. speciosa, but..., Flowers of 98 individuals of each of Phlox hirsuta and P. speciosa were collected from three populations where both species co-occur: China Hill, Greenhorn, and Soap Creek Ridge (Fig. 1).  Flowers were preserved in 100% EtOH. Flowers were dissected under a Nikon SMZ800N stereomicroscope, and images of the corolla, androecium, and gynoecium were captured using NIS-Elements software with a Nikon DS-Fi2 digital camera.  Using the NIS-Elements software, measurements were made for multiple floral features.
Leaf material of P. hirsuta was collected, in 2013, from 38-42 individuals in each of four putative populations – Cracker Gulch (CG), China Hill (CH), Greenhorn (G), Soap Creek Ridge (SC) (Fig. 1) – and in 2016, leaf material for P. speciosaand putative hybrids of the two species was collected from seven and four individuals from two populations (CH and SC), respectively.  The latitude and longitude were recorded for each collection. Leaf material was dried in silica gel and stored at 4° C..., , # Population genetics and comparative morphology of two serpentine Phlox species

[https://doi.org/10.5061/dryad.8931zcrt5](https://doi.org/10.5061/dryad.8931zcrt5)

## Description of the data and file structure

PHLOX_MICROSATS_STRUCTURE.txt is one of the STRUCTURE datasets used in the population genetic analyses. Samples are in the first column, population identifiers are in the second column, and the other column have allele lengths. The first row includes the names of loci. CG is Cracker Gulch, CH is China Hill, G is Greenhorn, and SC is Soap Creek Ridge. All individuals are members of Phlox hirsuta, except those labelled hybrid (putative hybrids) and P. speciosa, which are individuals of Phlox speciosa.

PHLOX_FLORAL_MORPHOLOGY.csv is the datafile used for morphometric analyses. The first row includes the samples and the measurements used (all in millimeters). Anther length, filament length, and filament attachment height collectively equal anther height, and ovary length, ...

稀有种与广布种之间的杂交可能导致稀有物种的遗传完整性丧失，这对物种管理与保护工作具有重要意义。其中一种稀有物种为多毛福禄考（*Phlox hirsuta*），它是加利福尼亚北部的蛇纹岩特有种，常与广布同属物种美丽福禄考（*P. speciosa*）伴生。研究人员基于中间型形态特征识别出疑似杂交个体，因此通过花部形态学与分子数据探究了杂交发生的可能性。研究从三个种群中各采集了98株多毛福禄考与美丽福禄考个体，并对其花部特征进行测量与比较。针对两个物种及疑似杂交个体扩增了11个微卫星（microsatellite）位点，并通过多种方法探究了种间与种内的遗传多样性及遗传关系。研究发现形态学与分子数据均存在变异：多毛福禄考的花部变异程度高于美丽福禄考；疑似杂交个体在遗传上与美丽福禄考亲缘关系更近，但……

研究从两个物种共同分布的三个种群——中国山（China Hill）、格林霍恩（Greenhorn）与皂溪岭（Soap Creek Ridge）（图1）——中各采集了98株多毛福禄考与美丽福禄考的花材。花材经100%乙醇（EtOH）保存。在尼康SMZ800N体式显微镜下对花部进行解剖，并通过搭载尼康DS-Fi2数码相机的NIS-Elements软件拍摄花冠、雄蕊群与雌蕊群的图像。随后使用NIS-Elements软件对多项花部特征进行测量。

2013年，研究人员从四个推定种群——克拉克峡谷（Cracker Gulch，CG）、中国山（CH）、格林霍恩（G）与皂溪岭（SC）（图1）——中各采集了38至42株多毛福禄考的叶片材料；2016年，从两个种群（CH与SC）中分别采集了7株美丽福禄考与4株两物种的疑似杂交个体的叶片材料。所有采样点均记录了经纬度坐标。叶片材料经硅胶干燥后保存于4℃环境中……

# 两种蛇纹岩福禄考的种群遗传学与比较形态学研究
https://doi.org/10.5061/dryad.8931zcrt5

## 数据与文件结构说明
`PHLOX_MICROSATS_STRUCTURE.txt`是种群遗传分析中使用的STRUCTURE数据集之一。该文件第一列为样本编号，第二列为种群标识符，其余列均为等位基因长度。第一行包含位点名称。其中CG代表克拉克峡谷，CH代表中国山，G代表格林霍恩，SC代表皂溪岭。除标注为杂交（疑似杂交）个体与*P. speciosa*（即美丽福禄考）的样本外，其余个体均为多毛福禄考。

`PHLOX_FLORAL_MORPHOLOGY.csv`是形态计量分析所用的数据文件。第一行包含样本名称与所用测量指标（单位均为毫米）。花药长度、花丝长度与花丝着生高度之和等于花药总高度，子房长度……