KLF2 and KLF4 in heart and lung endothelial cell transcriptional dynamics [ChIP-seq]. KLF2 and KLF4 in heart and lung endothelial cell transcriptional dynamics [ChIP-seq]

We report bulk RNA-sequencing, ChIP-seq, and ATAC-seq of endothelial cells harvested from heart and lung of multiple mouse strains investigating the role of KLF2 and KLF4 in endothelial transcription Overall design: Endothelial cells (ECs) were isolated from heart or lungs using flow cytometry (see below) and sujected to multiple sequencing modalities.

本研究报道了多株小鼠品系心脏及肺脏来源内皮细胞的批量RNA测序（bulk RNA-sequencing）、染色质免疫共沉淀测序（ChIP-seq）与转座酶可及性测序（ATAC-seq）数据，旨在探究KLF2与KLF4在内皮转录调控中的作用。整体实验设计：通过流式细胞术（flow cytometry）从心脏或肺脏中分离内皮细胞（ECs），并对其开展多种测序技术分析。