Single nuclei gene expression profile of brain cells from Pdgfbret/ret and Pdgfbret/wt mice

Brain calcification, the ectopic mineral deposits of calcium phosphate, is a common radiological finding in elderly and in various neurodegenerative disorders and a diagnostic criterion for primary familial brain calcification (PFBC). We made use of single nuclei RNA sequencing to understand the effect of calcification pathology in the Pdgfb (ret/ret) model of PFBC. Calcifications are located in deep brain regions - hypothalamus, thalamus, mid brain, pons of Pdgfb (ret/ret) mice and absent in control Pdgfb (ret/wt) mice. Forebrain cortex and hippocampus regions in both genotypes are calcification free. Detailed descriptions of the mouse model can be found in the following publications: Overall design: Nuclei were isolated from frozen brain tissue from cortical (calcification free) and deep brain (calcification prone) regions.

脑钙化（Brain calcification）指异位磷酸钙沉积，是老年人群及多种神经退行性疾病中常见的影像学表现，同时也是原发性家族性脑钙化（PFBC）的诊断标准。我们利用单细胞核RNA测序技术，解析钙化病理在PFBC的Pdgfb (ret/ret)小鼠模型中的作用。Pdgfb (ret/ret)小鼠的钙化沉积于脑深部区域，即下丘脑、丘脑、中脑及脑桥，而对照Pdgfb (ret/wt)小鼠无钙化现象。两种基因型小鼠的前脑皮层与海马体区域均无钙化沉积。该小鼠模型的详细描述可参阅以下已发表文献：实验设计：从皮层（无钙化区域）与脑深部（易钙化区域）的冻存脑组织中分离细胞核。