Molecular Signatures of Tumour and its Microenvironment for Precise Quantitative Diagnosis of Oral Squamous Cell Carcinoma: An Interna-tional Multi-cohort Diagnostic Validation Study

Supplementary Materials: The following supporting information can be downloaded at: www.mdpi.com/xxx/s1, Table ST1 – qMIDSV2 Gene panel primer sequences; Figure S1 – qMIDSV1 vs qMIDSV2 384-well assay format and protocols; Figure S2. Individual target gene expression pattern in 1761 samples; Figure S3. Various statistical methods used for gene selection analysis on 1761 clinical samples; Figure S4. Diagnostic performance comparison between qMIDSV2 vs qMIDSV2* (with 4 less effective genes removed from the panel of 14 target genes of qMIDSV2); Figure S5. Effect of removing individual genes from the 14-target gene panel qMIDSV2 (qV2) on diagnostic test performance based on the UK patient cohort data.

补充材料：以下支持信息可通过www.mdpi.com/xxx/s1下载，具体内容如下：表ST1——qMIDSV2基因组合引物序列；图S1——qMIDSV1与qMIDSV2的384孔检测体系及实验流程；图S2——1761份样本中各靶基因的表达模式；图S3——针对1761份临床样本开展基因筛选分析所采用的各类统计方法；图S4——qMIDSV2与qMIDSV2*的诊断性能对比（qMIDSV2*为从qMIDSV2的14个靶基因组合中剔除4个效能较低的基因后得到的版本）；图S5——基于英国患者队列数据，从14靶基因组合qMIDSV2（简称qV2）中逐一剔除单个基因对诊断检测性能的影响。