Cell autonomous and non-autonomous interactions of a western-style diet and the vitamin D receptor in intestinal homeostasis and tumorigenesis

These data suggest that co-culture with macrophages increases expression of NDRG-1 in epithelial cell lines. The finding is confirmed in 2 human epithelial cell lines, and in tissue derived from mice genetically and dietetically altered to increase macrophage infiltration of the small and large intestinal epithelium. NDRG1 is identified as a potential mediator of macrophage effects on tumorigenesis in the large and small intestine. Array data is part of a larger study involving the effects of Vitamin D, in concert with macrophages, on intestinal homeostasis and tumorigenesis. Cells from mouse epithelial cell line CT26 were cultured either alone, or with RAW macrophages in a system which allowed no physical contact but exchange of soluble factors between the cell types. The experiment was peformed twice.

本数据集相关数据表明，与巨噬细胞共培养可上调上皮细胞系中NDRG-1（NDRG-1）的表达水平。该结论在2株人源上皮细胞系以及经基因与饮食干预以提升巨噬细胞对小肠和大肠上皮浸润能力的小鼠组织样本中得到验证。NDRG1被鉴定为巨噬细胞调控大小肠肿瘤发生过程的潜在介质。本次芯片数据隶属于一项更大规模的研究课题，该课题旨在探究维生素D（Vitamin D）与巨噬细胞协同作用对肠道稳态及肿瘤发生的影响。实验采用小鼠上皮细胞系CT26（CT26）的细胞，分别进行单独培养，或与RAW巨噬细胞（RAW macrophages）在无细胞直接接触但可交换可溶性因子的体系中共培养。本实验共重复开展两次。