Biochemical and Metabolic Profiling of Amino Acids and Fatty Acids in Gracilaria corticata Polysaccharide-Treated Cells

This dataset presents the metabolic and biochemical profiling of cells treated with sulfated polysaccharides derived from Gracilaria corticata. It includes qPCR primer sequences and experimental conditions, physicochemical characterization of polysaccharide fractions (yield, molecular weight, and chemical composition), and quantitative analysis of intracellular metabolites. Specifically, the dataset provides relative abundance profiles of amino acids in HaCaT keratinocytes, RAW264.7 macrophages, and B16F10 melanoma cells, along with fatty acid composition in melanoma cells following treatment. These data support the evaluation of metabolic modulation and biochemical responses induced by G. corticata polysaccharides.本数据集涵盖经鸡冠江蓠（Gracilaria corticata）来源硫酸化多糖处理后的细胞代谢与生化谱分析数据，包含实时定量PCR（qPCR）引物序列、实验条件、多糖组分的理化表征数据（包括得率、分子量与化学组成），以及细胞内代谢物的定量分析结果。具体而言，本数据集提供了HaCaT角质形成细胞、RAW264.7巨噬细胞及B16F10黑色素瘤细胞内氨基酸的相对丰度谱数据，同时包含处理后黑色素瘤细胞的脂肪酸组成信息。上述数据可为评估鸡冠江蓠多糖诱导的代谢调控与生化应答反应提供支撑。