Primer set used for RT-PCR amplification of the eight vRNAs of influenza A viruses.

Note: the sequences complementary to the influenza sequences are shown in bold. The underlined nucleotides at the 3′-end represent the segment-specific sequences. The 5′-end has recognition sequences for the restriction endonuclease BsmBI.Primer set used for RT-PCR amplification of the eight vRNAs of influenza A viruses.注：与流感病毒序列互补的序列以粗体显示。3′端的下划线核苷酸代表片段特异性序列。5′端包含限制性内切酶BsmBI的识别位点。本引物组用于甲型流感病毒8种vRNAs的逆转录聚合酶链式反应（Reverse Transcription Polymerase Chain Reaction，RT-PCR）扩增。