Cell proliferation during whole-body regeneration in marine sponge Halisarca dujardinii (Demospongiae, Porifera). Part 1

Raw data on cell proliferation during whole-body regeneration (cell reaggregation) in demosponge Halisarca dujardinii for the manuscript: Lavrov et al. 'The role of cell proliferation and cell death in demosponge Halisarca dujardinii whole-body regeneration'.
Data are represented by CLSM Z-stacks (DAPI, EdU, pH3, ac-alpha-Tubulin) stored in .ims format. Z-stacks contains the original image and Imaris-generated spots used to count nuclei/EdU-positive nuclei.
This part of the dataset includes Z-stacks of the following reaggregation stages: 1) primary multicellular aggregates (PMA), 2) early-staged primmorphs (ESP), 3) primmorphs with oscular tube rudiments (OTR).
R scripts for statistical analysis are represented in RAR-archives.
Detailed protocols of the proliferation studies at all stage of cell reaggregation in H. dujardinii are represented in pdf-file.
Other parts of the dataset are available by Related links.

本数据集为Lavrov等人发表的论文《寻常海绵（demosponge）杜氏哈利斯卡海绵（Halisarca dujardinii）全身再生过程中的细胞增殖与细胞死亡作用》提供配套数据，包含该物种全身再生（细胞重聚过程）中的细胞增殖原始实验数据。数据以共聚焦激光扫描显微镜（Confocal Laser Scanning Microscopy, CLSM）Z轴堆叠图像形式存储，文件格式为.ims，成像通道涵盖DAPI、EdU、pH3、乙酰化α-微管蛋白（ac-alpha-Tubulin）。该Z轴堆叠图像包含原始成像结果与Imaris软件生成的、用于计数细胞核及EdU阳性细胞核的标记点集。本数据集涵盖以下细胞重聚阶段的Z轴堆叠图像：1）初级多细胞聚集体（primary multicellular aggregates, PMA）；2）早期原胚球（early-staged primmorphs, ESP）；3）带有出水口管原基（oscular tube rudiments, OTR）的原胚球。用于统计分析的R脚本已打包至RAR压缩档案中。杜氏哈利斯卡海绵（H. dujardinii）细胞重聚各阶段增殖研究的详细实验方案已整理为PDF文件。本数据集的其余部分可通过相关链接获取。