Table_1_Establishment of papillary thyroid cancer organoid lines from clinical specimens.xls

Papillary thyroid cancer (PTC) is a common malignancy of the endocrine system, and its morbidity and mortality are increasing year by year. Traditional two-dimensional culture of cell lines lacks tissue structure and is difficult to reflect the heterogeneity of tumors. The construction of mouse models is inefficient and time-consuming, which is difficult to be applied to individualized treatment on a large scale. Clinically relevant models that recapitulate the biology of their corresponding parental tumors are urgently needed. Based on clinical specimens of PTC, we have successfully established patient-derived organoids by exploring and optimizing the organoid culture system. These organoids have been cultured stably for more than 5 passages and successfully cryopreserved and retried. Histopathological and genome analysis revealed a high consistency of the histological architectures as well as mutational landscapes between the matched tumors and organoids. Here, we present a fully detailed method to derive PTC organoids from clinical specimens. Using this approach, we have developed PTC organoid lines from thyroid cancer samples with a success rate of 77.6% (38/49) until now.

甲状腺乳头状癌（Papillary thyroid cancer, PTC）是一种常见的内分泌系统恶性肿瘤，其发病率与死亡率逐年攀升。传统二维细胞系培养体系缺乏肿瘤组织结构，难以反映肿瘤的异质性；小鼠肿瘤模型的构建效率低下且耗时耗力，难以规模化应用于个体化治疗。目前亟需能够重现对应亲本肿瘤生物学特性的临床相关研究模型。本研究基于甲状腺乳头状癌临床标本，通过探索并优化类器官培养体系，成功构建了患者来源类器官（patient-derived organoids, PDOs）。该类器官可稳定传代至5代以上，且可成功冻存并复苏。组织病理学与基因组分析结果表明，配对肿瘤与其对应的类器官在组织学架构及突变谱上具有高度一致性。本文详细介绍了从临床标本中分离培养甲状腺乳头状癌类器官的完整方案。截至目前，利用该方法我们已从49例甲状腺癌样本中成功构建38株甲状腺乳头状癌类器官系，成功率达77.6%（38/49）。