WT and Slc5a8 knockout transcriptome analysis

Delineate the transcriptome profile of gut microbiota in wild type and Slc5a8-/- mice under low-fiber & high-fiber conditions to determine if transcriptome change occurs in the gut as a consequence of Slc5a8 deletion and/or low dietary fiber, thus driving the increased severity of colitis and enhanced colon cancer observed in Slc5a8-/- mice under low-fiber dietary conditions

本研究旨在解析野生型（wild type）与Slc5a8基因敲除（Slc5a8-/-）小鼠在低纤维、高纤维膳食条件下的肠道微生物群（gut microbiota）转录组谱（transcriptome profile），以明确肠道转录组的改变是否由Slc5a8基因缺失和/或膳食膳食纤维摄入不足所介导，并进一步探究该转录组变化是否会驱动Slc5a8-/-小鼠在低纤维膳食环境下出现结肠炎病情加重、结肠癌发生率显著升高的表型。