Skeletal muscle unloading affects muscle progenitor cells functional activity

In this work we employed classic skeletal muscle unloading rat model to determine how hindlimb suspension (HS) affects functional activity of skeletal muscle progenitor cells (SMPC). We have purified SMPC from m. soleus from control rats and after 1, 3, 7 and 14 days of exposure (HS1, HS3, HS7, HS14). Libraries were prepared with TruSeq Stranded mRNA kit, samples were RNA-sequenced on Illumina NextSeq 2000, read length 51 bp. Project was supported by RSF grant №20-75-10080.

本研究采用经典骨骼肌去负荷大鼠模型，探究后肢悬吊（hindlimb suspension, HS）对骨骼肌祖细胞（skeletal muscle progenitor cells, SMPC）功能活性的影响。我们从对照组大鼠以及暴露1、3、7、14天的后肢悬吊大鼠（分别记为HS1、HS3、HS7、HS14组）的比目鱼肌（m. soleus）中分离纯化了SMPC。采用TruSeq Stranded mRNA试剂盒构建文库，随后在Illumina NextSeq 2000测序平台上对样本进行RNA测序，测序读长为51 bp。本项目获得俄罗斯科学基金会（Russian Science Foundation, RSF）编号为20-75-10080的资助。