A novel chromatin tether domain controls topoisomerase IIα dynamics and mitotic chromosome formation

Shown are mitotic Muntiacus muntjac chromosomes immunostained to detect Topoisomerase IIα (green) at the axial core and histone H3 isoforms phosphorylated at Serine 28 (H3S28p; red). H3S28p localizes peripheral to Topo IIα and is largely excluded from the chromosome axial core. These localization patterns correlate with in vitro evidence that phosphorylation of Serine 28 inhibits binding of Topoisomerase IIα to histone H3.

本数据集展示了经免疫染色的有丝分裂赤麂（Muntiacus muntjac）染色体，用于检测轴芯处的拓扑异构酶IIα（Topoisomerase IIα，绿色荧光标记）以及丝氨酸28位点磷酸化的组蛋白H3亚型（H3S28p，红色荧光标记）。H3S28p定位于拓扑异构酶IIα的外周区域，且基本不分布于染色体轴芯内部。上述定位模式与体外实验所得证据相符——丝氨酸28的磷酸化会抑制拓扑异构酶IIα与组蛋白H3的结合。