Serum proteomics reveals high-affinity and convergent antibodies by tracking SARS-COV-2 hybrid immunity to emerging variants of concern

Dataset comprises of Ig transcript repertoire and proteomics data from three donors with hybrid immunity to SARS-CoV-2. The repertoires comprise of 10X Geneomics single-cell VDJ and bulk BCR sequencing of heavy and light chains, and are available in gzipped airrseq file format. Proteomics data was generated on an anti-Spike, anti-RBD, and flow-through fractions for each donor. Each fraction was split into heavy and light chains, and heavy chains were digested with chymotrypsin (chymo), elastase (elas), pepsin (peps), and trypsin (tryp), and analyzed with a 2hr MS/MS acquisition method on an ThermoFisher Eclipse Orbitrap instrument. Spectra are povided in tar gzipped MGF file format.本数据集包含3名具备新冠病毒（SARS-CoV-2）混合免疫的受试者的免疫球蛋白转录组库（Ig transcript repertoire）与蛋白质组学数据。该免疫组库涵盖针对重链与轻链的10X Geneomics单细胞VDJ测序及批量B细胞受体（BCR）测序结果，以经gzip压缩的AIRR-seq（airrseq）文件格式提供。蛋白质组学数据针对每名受试者的抗刺突蛋白、抗受体结合域（RBD）及穿透组分样本生成。每一组分均被拆分为重链与轻链组分，其中重链组分分别经胰凝乳蛋白酶（chymotrypsin，缩写chymo）、弹性蛋白酶（elastase，缩写elas）、胃蛋白酶（pepsin，缩写peps）及胰蛋白酶（trypsin，缩写tryp）酶解后，采用2小时串联质谱（MS/MS）采集方法，在赛默飞世尔（ThermoFisher）Eclipse Orbitrap质谱仪上完成分析。质谱光谱以经tar打包并gzip压缩的MGF文件格式提供。