GATA2 and progesterone receptor interaction in endometrial stromal cells undergoing decidualization [ChIP-Seq]

The zinc-finger transcription factor GATA2 has been shown to be important for endometrial stromal cell decidualization in early pregnancy in mice and humans. Progesterone and its receptor PGR is also critical during decidualization but its interaction with GATA2 in regulating genes and pathways necessary for decidualization in human endometrium are unclear. Human endometrial stromal cells were isolated from 5 premenopausal women for primary cell culture. The cells underwent in vitro decidualization (IVD) or vehicle (Veh) treatment for 10 days. RNA-sequencing (RNA-seq) was performed to compare gene expression profiles (n=3) and chromatin immunoprecipitation followed by sequencing (ChIP-seq) using an antibody against GATA2 (n=2) was performed to examine binding to target genes in the Veh and IVD cells. A public PGR ChIP-seq dataset (GSE69539) was mined to identify PGR-binding regions in IVD-treated human endometrial cells. Overall design: Genome-wide GATA2 binding profiling in human endometrial stromal cells with and without IVD treatment using ChIP-Seq

锌指转录因子GATA2（zinc-finger transcription factor GATA2）已被证实可在小鼠与人类早期妊娠过程中，对子宫内膜基质细胞的蜕膜化发挥关键调控作用。孕酮（Progesterone）及其受体PGR（progesterone receptor PGR）在蜕膜化过程中同样不可或缺，但二者在调控人类子宫内膜蜕膜化所需基因及通路的过程中存在何种相互作用，目前尚未明确。本研究从5名绝经前女性体内分离得到人子宫内膜基质细胞，用于原代细胞培养。将上述细胞分为两组，分别施以体外蜕膜化（in vitro decidualization, IVD）处理与溶剂对照（vehicle, Veh）处理，处理周期为10天。随后通过RNA测序（RNA-sequencing, RNA-seq）比较两组细胞的基因表达谱（n=3）；同时使用靶向GATA2的抗体开展染色质免疫共沉淀联合测序（chromatin immunoprecipitation followed by sequencing, ChIP-seq，n=2），以检测溶剂对照与体外蜕膜化处理组细胞中GATA2对靶基因的结合情况。此外，本研究还对公开的PGR ChIP-seq数据集（GSE69539）进行数据挖掘，以鉴定经IVD处理的人子宫内膜细胞中的PGR结合区域。整体实验设计：通过ChIP-seq技术，对经与未经IVD处理的人子宫内膜基质细胞开展全基因组范围内的GATA2结合谱分析。