Pairwise FST values for Aedes aegypti populations in Florida and southern California

In the affiliated paper we compare likely the oldest populations of Aedes aegypti in continental North America with some of the newest to illuminate the range of genetic diversity and structure that can be found within the invasive range of this important disease vector. Aedes aegypti populations in Florida have likely persisted since the 1600-1700s, while populations in southern California derive from new invasions that occurred in the last ten years. For this comparison, we genotyped 1,193 individuals from 29 sites at 12 highly variable microsatellites and a subset of these individuals at 23,961 single nucleotide polymorphisms (SNPs). This dataset contains the pairwise FST values generated with both genetic datasets.

Methods
We calculated pairwise genetic differentiation (FST) for microsatellites in Genepop v. 1.1.4. and tested for significance using an exact conditional contingency-table test with the following parameters: 10,000 dememorizations, 500 batches, and 10,000 iterations per batch. We calculated FST for SNPs using the same method, and we used 1000 permutations to test for significance in Arlequin v. 3.5.

For full descriptions of the genetic markers, see the affiliated manuscript or Dryad datasets.

本附属论文以北美大陆最古老的埃及伊蚊（Aedes aegypti）种群与部分新近种群为研究对象，旨在阐明这一重要疾病媒介昆虫入侵分布区内的遗传多样性与种群遗传结构特征。佛罗里达州的埃及伊蚊种群可能自17至18世纪便持续存续，而南加州的种群则源自近十年内发生的新入侵事件。为开展本次比较分析，我们对29个采样点的1193个个体进行了12个高多态性微卫星标记（microsatellites）的基因分型，并对其中部分个体开展了23961个单核苷酸多态性（SNPs）的基因分型。本数据集包含基于上述两套遗传标记数据集计算得到的两两FST值。
方法
我们使用Genepop v.1.1.4软件计算微卫星标记的两两遗传分化指数（FST），并采用精确条件列联表检验进行显著性验证，参数设置为：10000次消去迭代、500个批次以及每个批次10000次迭代。我们采用相同方法计算了SNP数据的FST值，并在Arlequin v.3.5软件中通过1000次置换检验完成显著性验证。
有关遗传标记的详细描述，请参阅附属论文或Dryad数据集。