Loss of ICOSL expression in the progression to cervical carcinoma

Human papillomavirus (HPV)-related lesions contain types with benign outcomes and those with a risk of progression to cancer. We addressed the role of immune surveillance in 76 cervical biopsies (normal = 23, HPV+ benign = 16, HPV+ precancer = 37) by studying the infiltration of cytotoxic T cells and the expression of the immune modulators PDL1, ICOSL, and miR-155, and compared the data to 101 cervical squamous cell carcinomas. In the normal cervix, ICOSL expression was restricted to the endocervical epithelia, whereas neither miR-155 nor PDL1 was detected. MiR-155 was up-regulated in both the benign (88%) and precancerous (92%) HPV squamous intraepithelial lesions (SIL) and co-localized to cells in the upper part of the lesion, that is, the area with productive viral infection. Both PDL1 (95%) and ICOSL (89%) were only evident in the precancerous SIL, and each localized to squamous cells in the basal aspect that lacked replicating virus. In both microinvasive and invasive cervical squa..., , # Loss of ICOSL expression in the progression to cervical carcinoma

Dataset DOI: [10.5061/dryad.2547d7x4g](10.5061/dryad.2547d7x4g)

## Description of the data and file structure

These are original raw images used in the \"Loss of ICOSL expression in the progression to cervical carcinoma manuscript, by Nuovo et al. They are in JPEG format, and the name of each file is self-explanatory. 

#### Description of the data and file structure

* The images are of cervical cancer patient samples, stained as indicated in their file names. In brief, samples from normal (healthy cervix, benign HPV-induced lesion, precancerous HPV-induced lesion, or high-grade cervical cancer) were stained for HPV-viral DNA, ICOSL, miR-155, ICOS, PDL1, PD1, and CD8, either alone or in co-expression analyses.  HPV in situ hybridization for viral RNA was done using the ACD RNAscope assay. Co-expression experiments for ICOSL and miR-155 were performed by analyzing a given slide with either DAB (brown signal), FAST RED...,

人乳头瘤病毒（Human papillomavirus, HPV）相关病变包含两类：一类为良性病变，另一类具有进展为癌症的风险。本研究针对76例宫颈活检样本（其中正常宫颈组织23例、HPV阳性良性病变16例、HPV阳性癌前病变37例），通过分析细胞毒性T细胞（cytotoxic T cells）浸润情况以及免疫调节因子PDL1、ICOSL和miR-155的表达水平，探讨免疫监视在此类病变中的作用，并将相关数据与101例宫颈鳞状细胞癌样本进行对比。在正常宫颈组织中，ICOSL的表达仅局限于宫颈内膜上皮，而未检测到miR-155与PDL1的表达。miR-155在良性（88%）与癌前（92%）HPV相关鳞状上皮内病变（squamous intraepithelial lesions, SIL）中均呈上调表达，且共定位于病变上部的细胞——即存在病毒活跃感染的区域。PDL1（95%）与ICOSL（89%）仅在癌前SIL中可被检测到，二者均定位于缺乏复制型病毒的基底侧鳞状细胞。在微浸润性与浸润性宫颈鳞状……，# 宫颈癌进展中ICOSL表达缺失

数据集DOI：10.5061/dryad.2547d7x4g

## 数据与文件结构说明

本数据集为Nuovo等人发表于"宫颈癌进展中ICOSL表达缺失"论文中使用的原始未处理图像，均采用JPEG格式存储，各文件名含义清晰直观。

#### 数据与文件结构说明
* 图像均来自宫颈癌患者样本，染色标记方式可通过文件名直观获知。简言之，研究涵盖正常（健康宫颈）、HPV诱导良性病变、HPV诱导癌前病变以及高级别宫颈癌四类样本，分别针对HPV病毒DNA、ICOSL、miR-155、ICOS、PDL1、PD1及CD8进行单染色或共表达分析。针对病毒RNA的HPV原位杂交实验采用ACD RNAscope技术完成。ICOSL与miR-155的共表达实验则通过对载玻片分别采用DAB（棕色信号）、FAST RED……进行分析完成。