Single-nuclei transcriptome profiling characterizes tumor microenvironment landscape in murine melanoma model. Mus musculus

Melanoma is one of the most aggressive forms of human tumors, although highly curable if diagnosed at an early phase. Despite many advances in targeted therapy and immunotherapy, the development of resistance remains a significant obstacle to melanoma curability, one important reason for this is tumor microenvironment (TME). Recent advances is single-nuclei RNA sequencing (snRNA-seq) have made it feasible to glean maximal information about cellular heterogeneity, thus making it possible to have a deep insight in the melanoma cancer cells and the TME. Nuclei were isolated from frozen tissue and snRNA-seq was performed on 4 samples, each coming from 2 different conditions (representing a biological replicate) - Start_Point and Tumor.

黑色素瘤（Melanoma）是人类恶性程度最高的肿瘤类型之一，但若能在早期阶段确诊，则治愈率极高。尽管靶向治疗与免疫治疗领域已取得诸多进展，但耐药性的产生仍是阻碍黑色素瘤治愈的关键障碍，其中一个重要原因便是肿瘤微环境（Tumor Microenvironment, TME）。近年来，单细胞核RNA测序（single-nuclei RNA sequencing, snRNA-seq）技术的进步使得全面获取细胞异质性相关信息成为可能，也为深入解析黑色素瘤细胞与肿瘤微环境提供了可行路径。研究人员从冷冻组织中分离细胞核，对4份样本开展单细胞核RNA测序，每份样本均对应两种不同的实验条件（即生物学重复样本）——Start_Point与Tumor。