Molecular controls over corticospinal neuron segmental target specificity. Sahni et al., Macklis

We FACS purified corticospinal neurons (CSN) in lateral versus medial cortex and analyzed genes differentially expressed between these subpopulations at three distinct developmental times - postnatal day 1 (P1), P4, and P7. The dataset was compiled using three biological replicates at P1, and two biological replicates at P4, and P7. The original microarray dataset is presented here along with gene lists of the top significantly differentially expressed genes in each subpopulation at P1, and P4. The excel files include the following: 1. The complete dataset showing gene intensities (normalized using rosetta resolver) comprising all probes, the normalized intensity for each probe in each biological dataset, the combined average intensity in each subpopulation at each time point, the fold change (of lateral versus medial CSN) at each time point, and the associated p value. 2. The top genes differentially expressed by lateral CSN at P1, ranked by SAM-RMA, along with the normalized rosetta intensities as described above. 3. The top genes differentially expressed by medial CSN at P1, ranked by SAM-RMA, along with the normalized rosetta intensities as described above. 4. The top genes differentially expressed by lateral CSN at P4, ranked by SAM-RMA, along with the normalized rosetta intensities as described above. 5. The top genes differentially expressed by medial CSN at P4, ranked by SAM-RMA, along with the normalized rosetta intensities as described above. The original microarray data has been deposited at the Gene Expression Omnibus database at NCBI (Accession GSE77311).

我们采用荧光激活细胞分选术（Fluorescence-Activated Cell Sorting, FACS）分离纯化了外侧皮层与内侧皮层中的皮质脊髓神经元（corticospinal neurons, CSN），并在三个不同的发育时点——出生后第1天（P1）、P4及P7——分析了这两类亚群间的差异表达基因。
本数据集的构建采用了P1时点的3份生物学重复样本，以及P4、P7时点各2份生物学重复样本。
本研究同步提供原始微阵列（microarray）数据集，以及各亚群在P1与P4时点的排名靠前的显著差异表达基因列表。
本次提供的Excel文件包含以下内容：
1. 完整数据集：涵盖所有探针的基因表达强度（经Rosetta Resolver标准化处理）、各生物学重复样本中每个探针的标准化强度、各时点各亚群的组合平均强度、各时点外侧与内侧皮质脊髓神经元的表达倍数变化，以及对应的显著性p值。
2. P1时点外侧皮质脊髓神经元的排名靠前的显著差异表达基因：按SAM-RMA方法进行排序，并附上前述的Rosetta标准化强度数据。
3. P1时点内侧皮质脊髓神经元的排名靠前的显著差异表达基因：按SAM-RMA方法进行排序，并附上前述的Rosetta标准化强度数据。
4. P4时点外侧皮质脊髓神经元的排名靠前的显著差异表达基因：按SAM-RMA方法进行排序，并附上前述的Rosetta标准化强度数据。
5. P4时点内侧皮质脊髓神经元的排名靠前的显著差异表达基因：按SAM-RMA方法进行排序，并附上前述的Rosetta标准化强度数据。
原始微阵列数据已提交至美国国家生物技术信息中心（National Center for Biotechnology Information, NCBI）的基因表达综合数据库（Gene Expression Omnibus, GEO），登录号为GSE77311。