miR-21-5p-driven gene expression in human colorectal DLD-1 cells.. miR-21-5p-driven gene expression in human colorectal DLD-1 cells.

Gene expression analysis of stable miR-21-5p-overexpressing DLD-1 cells (DLD-1-miR-21) and its control cells (DLD-1-miR-Vec). Overall design: To establish stable miR-21-overexpressing DLD-1 (DLD-1-miR-21) and corresponding vector-overexpressing (DLD-1-miR-Vec) cell lines, parental DLD-1 cells were transfected with a plasmid encoding human miR-21 or its control vector (pCMV-MIR) using the PolyJet transfection reagent. Stable clones were selected with 1 mg/mL G418 for 2 months. Single clones were selected based on expression of the green fluorescent protein (GFP) reporter and then pooled together for subsequent experiments.稳定过表达miR-21-5p的DLD-1细胞（DLD-1-miR-21）及其对照细胞（DLD-1-miR-Vec）的基因表达分析。整体实验设计：为构建稳定过表达miR-21的DLD-1细胞系（DLD-1-miR-21）及其对应的空载体对照细胞系（DLD-1-miR-Vec），将亲本DLD-1细胞采用PolyJet转染试剂转染编码人miR-21的质粒或其对照载体（pCMV-MIR）。使用1 mg/mL的G418筛选稳定克隆，筛选时长为2个月。基于绿色荧光蛋白（green fluorescent protein, GFP）报告基因的表达筛选单克隆，随后将单克隆混合后用于后续实验。