Identification and Characterization of the Epigenome and Transcriptome in Human Neonates. Identification and Characterization of the Epigenome and Transcriptome in Human Neonates

We performed reduced-representation bisulfite sequencing (RRBS) on 137 umbilical cord blood (UCB) samples and 158 parental peripheral blood (PPB) samples from nuclear families with either singletons or twins. The samples were classified into five groups based on the mode of conception: spontaneous (CTRL), IVF-ET, IVF-FET, ICSI-ET and ICSI-FET. There were no significant differences in the clinical features of the neonates among the different groups; in addition, maternal ages were under 35 years old and parental body mass index (BMI) values were comparable in general. In addition, we performed chromatin immunoprecipitation sequencing (ChIP-seq) on 33 UCB samples to examine histone modifications (H3K4me1, H3K4me3, H3K27ac, and H3K27me3) and RNA sequencing (RNA-seq) on 32 UCB samples to examine the transcriptomes of neonates. Overall design: RRBS, ChIP-seq (H3K4me1, H3K4me3, H3K27ac, H3K27me3), and RNA-seq in umbilical cord blood of neonates and peripheral blood of their parents.

本研究对来自单胎或双胎核心家庭的137份脐带血（umbilical cord blood, UCB）样本与158份父母外周血（parental peripheral blood, PPB）样本开展了简化代表性亚硫酸氢盐测序（reduced-representation bisulfite sequencing, RRBS）。本研究依据受孕方式将样本分为五组：自然受孕组（CTRL）、体外受精-胚胎移植（IVF-ET）组、体外受精-冻融胚胎移植（IVF-FET）组、卵胞浆内单精子注射-胚胎移植（ICSI-ET）组以及卵胞浆内单精子注射-冻融胚胎移植（ICSI-FET）组。各组新生儿的临床特征无显著组间差异；此外，所有研究对象的母亲年龄均小于35岁，父母的身体质量指数（body mass index, BMI）总体水平可比。此外，本研究对33份UCB样本开展染色质免疫沉淀测序（chromatin immunoprecipitation sequencing, ChIP-seq）以检测组蛋白修饰（H3K4me1、H3K4me3、H3K27ac及H3K27me3），并对32份UCB样本进行RNA测序（RNA-seq）以分析新生儿的转录组特征。研究整体设计：对新生儿脐带血及其父母外周血开展RRBS、ChIP-seq（检测H3K4me1、H3K4me3、H3K27ac及H3K27me3）以及RNA-seq实验。