Data_Sheet_1_An Eggplant Recombinant Inbred Population Allows the Discovery of Metabolic QTLs Controlling Fruit Nutritional Quality.xlsx

Eggplant (Solanum melongena L.) represents the third most important crop of the Solanaceae family and is an important component of our daily diet. A population of 164 F6 recombinant inbred lines (RILs), derived from two eggplant lines differing with respect to several key agronomic traits, “305E40” and “67/3,” was grown to the commercial maturation stage, and fruits were harvested, separated into peel and flesh, and subjected to liquid chromatography Liquid Chromatography/Mass Spectrometry (LC/MS) analysis. Through a combination of untargeted and targeted metabolomics approaches, a number of metabolites belonging to the glycoalkaloid, anthocyanin, and polyamine classes and showing a differential accumulation in the two parental lines and F1 hybrid were identified. Through metabolic profiling of the RILs, we identified several metabolomic quantitative trait loci (mQTLs) associated with the accumulation of those metabolites. Each of the metabolic traits proved to be controlled by one or more quantitative trait loci (QTLs); for most of the traits, one major mQTL (phenotypic variation explained [PVE] ≥ 10%) was identified. Data on mQTL mapping and dominance–recessivity relationships of measured compounds in the parental lines and F1 hybrid, as well as an analysis of the candidate genes underlying the QTLs and of their sequence differences in the two parental lines, suggested a series of candidate genes underlying the traits under study.

茄子（Solanum melongena L.）是茄科（Solanaceae）第三大重要作物，同时也是日常膳食的重要组成部分。以两个在多项关键农艺性状上存在差异的茄子品系"305E40"与"67/3"为亲本构建的164份F6代重组自交系（recombinant inbred lines, RILs）群体，被种植至商品成熟阶段后采收果实，将其分为果皮与果肉两部分，随后进行液相色谱-质谱联用（LC/MS）分析。通过非靶向与靶向代谢组学相结合的分析策略，本研究鉴定出多个属于糖生物碱、花青素及多胺类别的代谢物，这些代谢物在两个亲本品系与F1杂交种中呈现出差异积累特征。通过对该重组自交系群体进行代谢谱分析，本研究鉴定出多个与上述代谢物积累相关的代谢数量性状位点（metabolomic quantitative trait loci, mQTLs）。每一项代谢性状均由一个或多个数量性状位点（quantitative trait loci, QTLs）调控；对于大多数性状而言，均鉴定到了一个主效代谢数量性状位点，其解释的表型变异率（phenotypic variation explained, PVE）≥10%。结合代谢数量性状位点定位数据、供试化合物在亲本品系与F1杂交种中的显隐性关系分析，以及对数量性状位点侧翼候选基因及其在两个亲本品系间序列差异的分析，本研究提出了一系列与所研究性状相关的候选基因。